The Establishment of In vitro Cultures from Mature Khinjuk Pistachio (Pistacia khinjuk Stocks) Trees
Abstract: Today, there are many in vitro micropropagation studies related to the pistachio rootstocks. However rootstock propagation still made with seeds the cause of difficulties to start in vitro cultures from mature trees with desired characteristics. The contamination, phenolic exudation and browning problems observed in explants taken from mature rootstocks are the biggest obstacles to the start of the in vitro cultures. In this study, the aim was to develop an in vitro culture initiation methods for mature female khinjuk pistachio (Pistacia khinjuk Stocks) using 1-2 cm long apical or lateral buds. Phenolic compounds that exudated from the explants were removed without using any adsorbent compound rate of 92%. As a result of study, the best living and sprouted explant rate (32.5%) were obtained from apical shoot tips by treating for 30 minutes with 10% NaOCl.
Keywords: Plant tissue culture, Khinjuk pistachio, Micropropagation, Sodium Hypochlorite