Batman Universitesi Yasam Bilimleri Dergisi
www.yasambilimleridergisi.com
Cilt 1, Sayı 2  Ocak-Haziran 2012  (ISSN: 2147-4877, E-ISSN: 2459-0614)
Yelda Özden Çiftçi, Hülya Akdemir, Veysel Süzerer, Engin Tilkat, Ahmet Onay

NO Makale Adı
1356122718 Medium-Term In Vitro Storage of Mature Pistachio Microshoots

The most common way of in vitro conservation of plant species concerns the
usage of slow growth storage techniques in which temperature is reduced together
with decrease of light intensity. Thus, in vitro proliferated microshoots of mature male
“Atlı” and female “Siirt” were conserved in low temperature in dark for up to 12 months
in this study in order to reveal out optimum storage period. Following the transfer of
shoot apices excised from conserved microshoots to standard in vitro conditions, 97%
proliferation was achieved even after the longest tested storage period. In addition,
2.3 and 2.6 microshoots per explant were also obtained in “Atlı” and “Siirt” cultures,
respectively, with 12 months of storage. The relatively greater proliferation rates together
with the higher number of shoots proliferated per explant indicated that in vitro-grown
pistachio microshoots could be stored for longer period at low temperature without
requiring subculture. The optimized technique has potential to be used for development
of effective medium-term conservation protocols for other Pistacia species.